A fully funded 2-year postdoc position is available at the laboratory of protein design (https://vorobievalab.sites.vib.be/en) at the VIB-Vrije Universiteit Brussel Center of Structural Biology, Brussels, Belgium. The project is interdisciplinary (computational/wet lab work) and aims to design and test novel transmembrane beta-barrel pores for single-molecule sensing applications.
We recently developed new computational methods to design entirely from physical principles transmembrane beta-barrels (Vorobieva et al., Science 2021). The beta-barrels insert into lipid membranes and stably conduct ions. Here, we aim to design defined sensing functions into the synthetic nanopore proteins.
The tasks will include computational design, production and purification from E. coli inclusion bodies, biophysical characterization in detergent micelles, synthetic lipid membranes and single-channel recording. The structure of the most promising nanopores will be determined by Cryo-EM or crystallography.
Profile
Candidates should hold a Ph.D. in Sciences (eg. Biochemistry, Biophysics) or Bio-engineering sciences and have a strong background in recombinant expression, purification and handling of membrane proteins in detergent micelles and synthetic lipid membranes. Experience in single-channel recording, python scripting and/or High Performance Computing is desired. The candidate should have a keen interest in protein design and an engineering-oriented mindset. Experience with research valorization and IP will be regarded as a plus.
How to apply?
Please apply online with a cover letter (in English) and your CV including and the contact information of 2 referees.
For further information please contact Dr. Anastassia Vorobieva ([email protected]).
Desired starting date: 1st of April 2024 or earlier
